热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

所有图片（1/2）

UMP-CMP kinase/CMPK1 His Tag Protein, Human

CK,CMK,CMPK,UMK,UMP-CMPK,UMPK

价格 ￥1,950.00 （ 1-2周）
货号 UA070016
规格
数量
收藏 分享

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    CK,CMK,CMPK,UMK,UMP-CMPK,UMPK

  • Accession

    P30085

  • 表达序列

    Met1-Gly196, with N-terminal 8*His

  • 表达宿主

    Baculovirus-InsectCells

  • 分子量

    26-29kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 活性

    0.010µg/mL - 0.013µg/mL

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    20mM Tris, 500mM NaCl,10%Glycerol, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Liou J. et al. (2004) Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium. Molecular Pharmacology. 67(3): 806-14.

    2、Gerhard D S. et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Research. 14(10B): 2121-7.

    3、Segura-Pena D, et al. (2004) Substrate-induced Conformational Changes in Human UMP/CMP Kinase. Journal of Biological Chemistry. 279(32): 33882-9.

  • 稀释度

背景介绍

  • Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. The gene of encodes one of the enzymes required for cellular nucleic acid biosynthesis. CMPK1 plays an important role in de novo pyrimidine nucleotide biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer. It has 3 isoforms with the molecular mass of 22, 16 and 26 kDa. The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD.

  • 生物活性

    • When UMP-CMP kinase/CMPK1 His Tag, Human is immobilized at 0.2µg/mL, it binds to rabbit anti UMP-CMP kinase pAb with an EC50 of 0.010 to 0.013µg/ml.

  • 电泳

    • 2μg (R: reducing conditions).

    评论(0)