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物种
Serratia marcescens分子别名
SMNE,Nuclease,UltraNuclease,Benzonase表达序列
Asp22-Asn266
DTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN
表达宿主
E.coli分子量
27.7kDa (Reducing)
纯度
＞95% by SDS-PAGE
活性
250U/μL标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol
储存条件
· 12 months from date of receipt, -20 to -70 °C as supplied.
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Nestle M, Roberts W K. An Extracellular Nuclease from Serratia marcescens I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME[J]. Journal of Biological Chemistry, 1969, 244.
2.Kim W Y, Lee H S, Suh S J, et al. Purification and Cellular Localization of Extracellular Nuclease of Serratia marcescens Expressed in Escherichia coli[J]. Korean Journal of Microbiology, 1994, 32(2):147-154.
Multi Nuclease all-around nuclease, also called broad-spectrum nucleic acid enzyme, is a kind of comes from Serratia Marcescens restriction endonuclease. It is capable of degradation of all forms of DNA and RNA (double-stranded, single-stranded, linear, circular or superhelical forms) under a very wide range of conditions (6Murea, 0.1M GuanidineHCl, 0.4%TritonX100, 0.1%SDS, 1mM EDTA, 1mM PMSF). The formation of 3-5 oligonucleotide residues containing 5 '-phosphate terminus is widely used to remove nucleic acids from biological products. The expression and purification of this product in Escherichia coli(E.coli) through genetic engineering can not only reduce the viscosity of cell supernatant and cell lysate in scientific research, but also improve the efficiency of protein purification and functional research. It can also be used in virus purification, vaccine production, protein and polysaccharide pharmaceutical industry as a host residual nucleic acid removal reagent, reducing the host residual nucleic acid to the peak (pg) level to improve the efficacy and safety of biological products. And can effectively prevent human peripheral blood monocyte (PBMC) clumping in cell therapy and vaccine research.
Components
Amount
UltraNuclease *
250U/μL
Buffer Formulation
10mM Tris (pH7.4), 500mM NaCl, 2mM MgCl2, 50% glycerol
* One unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min.
One unit of Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min
生物活性
- M marker1:Expi 293 Cell Lysis solution A(ultrasonicated).2:Expi 293 Cell Lysis solution B( non-ultrasonicated).3:Expi 293 Cell Lysis solution A+0.5μl UltraNuclease.4:Expi 293 Cell Lysis solution B+0.5μl UltraNuclease.5:Expi 293 Cell Lysis solution A+1μl UltraNuclease.6:Expi 293 Cell Lysis solution B+1μl UltraNuclease.Reaction condition: Incubate at 37℃ for 30mins.
- M marker 1:0.2g/ml Escherichia coli lysate.2:0.2g/ml Escherichia coli lysate+1μl well-known UltraNuclease.3:0.2g/ml Escherichia coli lysate+1μl UltraNuclease.
- M marker 1:10μg Salmon sperm DNA.2:37μg Salmon sperm DNA+0.5U UltraNuclease.3:37μg Salmon sperm DNA+0.75U UltraNuclease.4:37μg Salmon sperm DNA+1U UltraNuclease.
- M marker 1:plasmid A.2:plasmid A+ UltraNuclease.3:plasmid B.4:plasmid B+ UltraNuclease.
电泳
- Complement UltraNuclease,1µg on SDS-PAGE under reducing and Non-reducing condition. The Purity is greater than 95%.
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