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物种
Ebola virus分子别名
bovGP; EBOV GP; second secreted glycoprotein; small secreted glycoprotein; Spike glycoproteinAccession
AHX24649表达序列
Ile33-Gln650 with His Tag at C-Terminus
表达宿主
Baculovirus-InsectCells分子量
Cleaved partially by a furin-like convertase to GP1 and GP2 with predicted molecular mass of 50.9 KDa and 18.4 kDa. As a result of glycosylation, it migrates as an approximately 72-95 KDa and 20-25 kDa band in SDS
纯度
＞95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4,5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
2.de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
The glycoprotein (GP) encoded by the Ebola virus genome is a critical determinant of the pathogenicity of Ebolavirus hemorrhagic fever viruses. It is processed into distinct forms for incorporation into the virus envelope, presentation on the cell surface, or release from infected cells. GP is a class I fusion protein that assembles as trimers on the viral surface, playing a crucial role in virus attachment and entry. The mature GP is a disulfide-linked heterodimer composed of two subunits, GP1 and GP2, generated from the proteolytic processing of the GP precursor (pre-GP) by cellular furin during virus assembly.
GP1 binds to receptors on target cells, interacting with CD209/DC-SIGN and CLEC4M/DC-SIGNR, which serve as cofactors for virus entry into host cells. GP2 functions as a class I viral fusion protein. The GP1,2 heterodimer mediates endothelial cell activation and reduces endothelial barrier function. Soluble GP (sGP) appears to have anti-inflammatory activity, as it can reverse the barrier-disrupting effects of TNF-alpha.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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