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物种
Human分子别名
CD22, BL-CAMAccession
P20273-1表达序列
Asp20-Arg687, with C-terminal 10*His
表达宿主
HEK293分子量
95-120kDa (Reducing)
标记
Alexa Fluor® 647标签
His Tag性状
Liquid缓冲体系
PBS, PH7.4
储存条件
· 12 months from date of receipt stored at --20 to -80℃.
· 1 week, 2 to 8℃ under sterile conditions
· Recommend to aliquot the protein into smaller quantities for optimal storage.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Tedder T F. et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
2. Fujimoto M. et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
3. Walker J A. et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-325.
4. Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-143.
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Siglec-2 (CD22) belongs to a family of immune inhibitory Siglecs, which bears ITIM to deliver immunosuppressive signals such as reduced phagocytosis, dampened inflammation, and inhibited danger-associated molecular pattern/ pathogen-associated molecular pattern (DAMP/PAMP)-mediated inflammation. Siglec-2 is a cell surface receptor expressed mostly on B cells that regulates B-cell proliferation, survival, signaling, and antibody production. The structure of Siglec-2 contains six tyrosines in its cytoplasmic tail, four of which are in ITIM motifs: Y783, Y843, Y863, and Y828. Following cross-linking of B-cell receptors (BCRs), these tyrosine residues are phosphorylated and recruit Src homology region 2 (SH2) domain-containing protein tyrosine phosphatase-1 (SHP-1), which dephosphorylates BCR-proximal signaling complexes. Siglec-2 is an attractive therapeutic target considering its unique presence in B lymphocytes. Currently, many ongoing trials evaluating CD22-directed chimeric antigen receptors (CARs), particularly in children with relapsed or refractory B-cell leukemia, are showing safety and efficacy. Some novel CARs, such as bispecific CD20/CD22 CARs and CD19/CD22 CARs, are also in development.
流式分析
5e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Alexa Fluor 647 Labeled-Siglec-2 His Tag Protein, Human, (Cat. No. UA011196) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity.
5e5 of transient transfected anti-Siglec-2 ScFv CAR-293 cells were stained with 0.1ug Siglec-2 His Tag Protein, Human (Cat. No. UA010679) and competitor respectively (Fig. D and E). APC signal was used to evaluate the binding activity.
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