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物种
Human分子别名
IL-4R alpha,CD124,IL4R,IL4RAAccession
P24394表达序列
Met26-His232, with C-terminal 8*His
表达宿主
HEK293分子量
36-54kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Nelms K. et al. (2003) The IL-4 receptor: signaling mechanisms and biologic functions. Annual Review of Immunology. 17: 701-738.
IL-4Rα is a member of the hematopoietin receptor superfamily. Among the defining features of the members of this superfamily of receptors are shared structural motifs in the extracellular region, which consists of type III fibronectin domains. These motifs include conserved paired Cys residues and, in the membrane proximal region, a WSXWS motif. The latter has been proposed to be required for maintaining the receptor in a conformation favorable to cytokine binding. Structural alterations in the IL-4Rα extracellular region may result in altered receptor signaling capabilities. Indeed, a variant of the human IL-4Rα chain containing a Ile50Val substitution was isolated from atopic individuals and has been shown to enhance signal transduction resulting in the increased production of IgE.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) at 2.0μg/mL (100μL/well) can bind Anti-CD124 Monoclonal Antibody(Dupilumab) (Cat. No. S0B0560) with EC50 of 2.41-2.98ng/mL.
Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) with EC50 of 13.41-18.20ng/mL.
Immobilized IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) at 1.0μg/mL (100μL/well) can bind IL-4 Fc Chimera Protein, Human with EC50 of 0.49-1.08ng/mL.
The ELISA based assay shows that IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) is stable in the quality of different batches.
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