StepSlim Human IFN-gamma（ALP）ELISpot Kit

Stimulate 1×10⁵ cells/well of cryopreserved PBMCs with PHA. After 20 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.

Linearity
Six different cryopreservation PBMCs concentrations, 4 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the six cell concentrations

Precision
Stimulate 1×10⁵ cells/well of cryopreservation PBMCs with PHA for 20 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation