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抗体类型
Recombinant mAb应用
mIHC反应种属 ?
Hu标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| mIHC | 1:200-1:1000 |
PD-1, T cell, Cytotoxic T cell and Macrophage Marker (PD-1, CD3 epsilon, CD8α, CD68) Antibody Panel contains 4 recombinant monoclonal antibodies against PD-1, CD3 epsilon, CD8α, CD68. They are provided as a sampler panel.
Panel contains:
- S-RMab® PD-1 Recombinant Mouse mAb (SDT-R143) S0B2187
- CD3 epsilon Recombinant Rabbit mAb (SDT-241-49) S0B2132
- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148
- S-RMab® CD68 Recombinant Rabbit mAb (SDT-R025) S0B2064
组分和操作说明
多重免疫组化
mIHC shows positive staining in paraffin-embedded human tonsil (A). Anti- CD3 epsilon Rabbit mAb (S0B2132) was used at 1/1000 dilution (Green, B), Anti-CD8α Mouse mAb (S0B2148) was used at 1/500 dilution (Orange, C), Anti- PD-1 Mouse mAb (S0B2187) was used at 1/200 dilution (White, D), and Anti-CD68 Rabbit mAb (S0B2064) was used at 1/500 dilution (magenta, E), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.
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