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Rabbit抗原名称
Phospho-Stat5 (Tyr694)分子别名
Signal transducer and activator of transcription 5A; STAT5; STAT5A免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P42229克隆号
S-1183-58抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM反应种属 ?
Hu阳性样本
IL-2-treated Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Phospho-Stat5 (Tyr694) is the transcription factor Signal Transducer and Activator of Transcription 5 that has undergone phosphorylation on tyrosine residue 694 within its C-terminal SH2 domain; this single, cytokine-activated modification induces Stat5 dimerization, nuclear translocation, and sequence-specific DNA binding to GAS elements, thereby driving transcription of genes controlling proliferation, survival, erythropoiesis, lactation, and immune regulation, with aberrant pY694-Stat5 signaling being a hallmark of several hematologic malignancies and solid tumors where it sustains oncogenic gene expression programs.
流式分析
Flow cytometric analysis of Phospho-Stat5 (Tyr694) expression on IL-2-treated Human PBMC. Human PBMC (peripheral blood mononuclear cells) treated 15min with IL-2 were stained with either Alexa Fluor® 647 Rabbit IgG Isotype Control (Left panel) or SDT Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb (A647 Conjugate) (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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