GST Tag Recombinant Rabbit mAb (Alexa Fluor^® 647 Conjugate) (S-372-19)

The GST tag protein is an extremely important tool in biotechnology. GST stands for Glutathione S-transferase, an enzyme widely present in cells. It plays a key role in various metabolic processes, especially in detoxification. In the field of biotechnology, the GST gene is often used as a fusion tag gene, which is fused with the target protein gene to form a GST fusion protein. This fusion protein has many advantages. Firstly, GST itself has high solubility, which can help increase the solubility of the target protein. This prevents the target protein from forming inclusion bodies during expression, making it easier for subsequent purification operations. Secondly, GST fusion proteins can be rapidly and efficiently purified using specific ligands such as glutathione agarose beads. The purification process is relatively simple and cost-effective. Moreover, in many cases, the GST tag does not significantly affect the structure and function of the target protein. Additionally, it can be removed by specific enzymatic cleavage sites when pure target protein is needed. Therefore, the GST tag protein is widely used in many fields, such as recombinant protein expression, structural studies, antibody preparation, and protein-protein interaction research, greatly facilitating life science research.