Acetyl-CoA Carboxylase Recombinant Rabbit mAb (S-2220-96)

Acetyl-CoA carboxylase 1,ACC1,Acetyl-Coenzyme A carboxylase alpha (ACC-alpha),ACAC,ACCA,ACACA

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6078

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Acetyl-CoA Carboxylase
分子别名

Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha (ACC-alpha); ACAC; ACCA; ACACA
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q13085
克隆号

S-2220-96
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, A431, HeLa, HepG2, NIH/3T3, mouse brain, C6, rat brain
预测反应种属
(反应种属缩写表)

Bv, Ck, Sh
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Ms
ICFCM 1:50 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Ms
ICFCM	1:50	Ms

背景介绍

Acetyl-CoA Carboxylase (ACC) is a crucial metabolic enzyme that plays a central role in fatty acid metabolism. It catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC exists in two isoforms: ACC1 (ACACA), primarily localized in the cytosol and regulating fatty acid synthesis, and ACC2 (ACACB), which is associated with the outer mitochondrial membrane. The malonyl-CoA produced by ACC2 inhibits carnitine palmitoyltransferase-1 (CPT-1), thereby regulating fatty acid β-oxidation. The enzyme is tightly controlled by multiple mechanisms, including allosteric regulation (activation by citrate, inhibition by long-chain acyl-CoA), reversible phosphorylation (inactivation by AMPK and PKA), and transcriptional regulation (e.g., SREBP-1c-mediated expression upregulation). Due to its pivotal role in energy metabolism, ACC is a potential therapeutic target for obesity, diabetes, and cancer, with inhibitors (e.g., TOFA, ND-630) currently under investigation. Additionally, plant and bacterial ACCs are targets for herbicides (e.g., aryloxyphenoxypropionates) and antibiotics, highlighting their broad biological significance.

免疫印迹
- WB result of Acetyl-CoA Carboxylase Recombinant Rabbit mAb
  Primary antibody: Acetyl-CoA Carboxylase Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 266 kDa
  Observed MW: 266 kDa
- WB result of Acetyl-CoA Carboxylase Recombinant Rabbit mAb
  Primary antibody: Acetyl-CoA Carboxylase Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 266 kDa
  Observed MW: 266 kDa
- WB result of Acetyl-CoA Carboxylase Recombinant Rabbit mAb
  Primary antibody: Acetyl-CoA Carboxylase Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 266 kDa
  Observed MW: 266 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Acetyl-CoA antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti- Acetyl-CoA Carboxylase antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Acetyl-CoA Carboxylase antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Acetyl-CoA Carboxylase antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti- Acetyl-CoA Carboxylase antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in NIH/3T3 cells. Anti- Acetyl-CoA Carboxylase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Acetyl-CoA Carboxylase in tumor tissue.
- Expression of Acetyl-CoA Carboxylase in human tissue.
- Expression of Acetyl-CoA Carboxylase in mouse & rat tissue.