Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)
Rabbit IgG,Rabbit Immunoglobulin G,Rabbit IgG Heavy Chain,Rabbit IgG γ,Rabbit IgGγ,Rabbit IgG gamma,Rabbit IgG Light Chain,Rabbit IgG κ,Rabbit IgGκ,Rabbit IgG kappa,Rabbit IgG λ,Rabbit IgGλ,Rabbit IgG lambda,Rabbit IgG Fc,Rabbit IgG Fab,Rabbit IgG (H+L),rabbit IgG
货号： S0B4040
- 价格： ￥1,856
- 规格：
- 数量：
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宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody应用
IF反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IF | 1:500 | Rb |
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment is a type of secondary antibody fragment that is widely utilized in various immunological assays due to its specific characteristics. Derived from immunoglobulins of goats that have been immunized against rabbit IgG, this fragment consists of the antigen-binding regions (Fab')2 of the antibody molecule, which includes both the heavy (H) and light (L) chains. The F(ab')2 fragment is obtained by enzymatic digestion of the whole IgG molecule, specifically using pepsin, which cleaves the molecule into pieces while retaining the antigen-binding sites.
免疫荧光
IF shows positive staining in paraffin-embedded human bladder cancer. Anti-p21 Waf1/Cip1 antibody (S0B2351) was used at 1/100 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- p21 Waf1/Cip1 antibody (S0B2351) was used at 1/100 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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