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宿主来源
Goat抗原名称
Mouse IgG抗体类型
Polyclonal antibody应用
FCM ,IF ?稀释度
应用 稀释度 推荐种属 IF 1:500 Ms FCM 1:2000 Ms 反应种属 ?
Ms纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Goat Anti-Mouse IgG (H+L), F(ab')2 Fragment is a type of secondary antibody that is widely used in various immunoassays due to its specific binding properties. This antibody fragment is derived from the immunoglobulin of goats that have been immunized against mouse immunoglobulins. The "H+L" in the name indicates that the fragment includes both the heavy (H) and light (L) chains of the immunoglobulin. The F(ab')2 fragment is created by enzymatic digestion of the whole IgG molecule, which removes most of the Fc region, the part of the antibody that is responsible for effector functions and binding to Fc receptors on cells.
免疫荧光
IF shows positive staining in paraffin-embedded human ovarian cancer. Anti-MUC1 antibody (S0B2293) was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human kidney. Anti-MUC1 antibody (S0B2293) was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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