CD3 epsilon Recombinant Rabbit mAb (SDT-241-49)

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货号： S0B2132

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

CD3 epsilon
分子别名

T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3E, CD3E
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P07766
克隆号

SDT-241-49
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB, IP, IF
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Rb, Mq, Pg, Sh
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:250-1:500
IP	1:25
ICC	1:250
IF	1:1000

背景介绍

CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.

免疫印迹
- WB result of CD3 epsilon Rabbit mAb
  Primary antibody: CD3 epsilon Rabbit mAb at 1/500 dilution
  Lane 1: Raji whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Negative control: Raji whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 20 kDa
  Observed MW: 20 kDa
免疫沉淀
- CD3 epsilon Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating CD3 epsilon in 0.4mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD3 epsilon Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1 : Jurkat whole cell lysate 10µg(input)
  Lane 2 : CD3 epsilon Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3 : Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 20 kDa
  Observed MW: 20 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti-CD3 epsilon antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-CD3 epsilon antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD3 epsilon antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.