热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 病理/IHC抗体

所有图片（1/11）
申请试用

Vimentin Recombinant Rabbit mAb (SDT-029-20)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2004
规格
数量
收藏 分享

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Vimentin

  • 免疫原

    Synthetic Peptide

  • Accession

    P08670

  • 克隆号

    SDT-029-20

  • 抗体类型

    Rabbit mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB ,
    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:2000-1:10000 Hu, Ms
    IHC-P 1:1000 Hu, Ms
    ICC 1:200 Hu, Ms, Rt
    IF 1:200 Hu

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Pr, Bv, Cz, Pg, Hm, Av

  • 纯化方式

    Protein A

  • 研究领域

    Neuroscience

  • 浓度

    1 mg/ml

  • 分子量

    56kDa

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

背景介绍

  • Vimentin is a structural protein that in humans is encoded by the VIM gene. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

  • 免疫印迹

    • WB result of anti-Vimentin antibody

      Primary antibody : Anti-Vimentin antibody at 1:2000 dilution
      Lane 1 : Daudi whole cell lysate 20 µg
      Lane 2 : Hela whole cell lysate 20 µg
      Lane 3 : A549 whole cell lysate 20 µg
      Lane 4 : Jurkat whole cell lysate 20 µg
      Negative control: Daudi whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution


      Predicted MW: 54 kDa
      Observed MW: 57 kDa

    • WB result of anti-Vimentin antibody

      Primary antibody : Anti-Vimentin antibody at 1:2000 dilution
      Lane 1 : NIH/3T3 whole cell lysate 20 µg


      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution


      Predicted MW: 54 kDa
      Observed MW: 57 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded stromal of human colon cancer.

      Anti-Vimentin antibody was used at 1:1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded stromal of human breast cancer.

      Anti-Vimentin antibody was used at 1:1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded stromal of mouse colon. Anti-Vimentin antibody was used at 1:1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in NIH/3T3 cells. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in C6 cells. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human breast cancer. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human colon cancer. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human prostate cancer. Anti-Vimentin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

评论(0)