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His tag Recombinant Rabbit mAb (HRP Conjugate) (S-1398-151)

价格 ￥550.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1842
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    His tag

  • 免疫原

    Synthetic Peptide

  • 克隆号

    S-1398-151

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Species Independent

  • 阳性样本

    293T transfected with His-tagged fusion protein

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:5000-1:10000 Species Independent
背景介绍

  • A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. This antibody recognizes His-tags placed at N-terminal and C-terminal of fusion proteins.

  • 免疫印迹

    • WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
      Primary antibody: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 1: 293T transfected with His-tagged fusion protein whole cell lysate 4 µg
      Predicted MW: 17 kDa
      Observed MW: 17 kDa

    • WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
      Primary antibody: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 1: Angiopoietin-1(277-498) His Tag Protein, Mouse 20 ng
      Lane 2: Angiopoietin-2(275-496) His Tag Protein, Human 20 ng
      Predicted MW: 28-33 kDa
      Observed MW: 30 kDa

    • WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
      Primary antibody: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 1: 293T whole cell lysate 4 µg
      Lane 2: 293T transfected with His-tagged fusion protein whole cell lysate 4 µg
      Predicted MW: 17 kDa
      Observed MW: 17 kDa

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