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Rabbit抗原名称
MBP tag分子别名
Maltose-binding protein免疫原
Synthetic PeptideAccession
P0AEX9克隆号
S-925-33抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Species Independent纯化方式
Protein A浓度
2 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000-1:10000 | Species Independent |
Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest is often cleaved from MBP with a specific protease and can then be separated from MBP by affinity chromatography.
免疫印迹
WB result of MBP tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: 293T transfected with Histone H3-MBP tag fusion protein whole cell lysate 20 µg
Primary antibody:
Lane 1: MBP tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: MBP tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: MBP tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Predicted MW: 43 kDa
Observed MW: 60 kDa
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