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Rabbit抗原名称
PD-1分子别名
CD279, PDCD1免疫原
Synthetic Peptide细胞定位
MembraneAccession
Q15116克隆号
SDT-035-25抗体类型
Recombinant mAb抗体同种型
IgG应用
IF ?反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 555性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IF | 1:200 | Hu |
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil (case 1). Anti-PD-1 (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated 45min at RT. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human tonsil (case 2). Anti-PD-1 (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated 45min at RT. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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