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FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) (S-589-8)

价格 ￥480.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1648
规格
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产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    FLAG Tag

  • 免疫原

    Synthetic Peptide

  • 克隆号

    S-589-8

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:5000-1:10000 Species Independent

  • 反应种属 ?

    Species Independent

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    HRP

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

背景介绍

  • FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

  • 免疫印迹

    • WB result of FLAG Tag Recombinant Rabbit mAb (HRP Conjugate)
      Lysate: 293T transfected with Histone H3-Flag-V5 whole cell lysate 20 µg
      Primary antibody:
      Lane 1: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
      Lane 2: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
      Lane 3: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
      Lane 4: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

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