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Rabbit抗原名称
S100B分子别名
Protein S100-B, S-100 protein beta chain免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, SecretedAccession
P04271克隆号
SDT-518-45抗体类型
Recombinant mAb抗体同种型
IgG应用
IF ?反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:100 |
S100B is a Ca2+-binding protein, which is secreted from astrocytes. S100B is widely expressed in astrocytes, certain neuronal populations, Schwann cells, myeloid-derived cells, and a few other cell types. In diagnostic pathology, S100B – also termed “S100” – is used as a marker for Schwann cells and melanocytes. In these cell types, S100B is highly expressed, however, S100B is also regularly seen in various other cell types. It is mainly used for differentiated diagnosis of malignant melanoma, cartilagea, and neurological tumors.
免疫荧光
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-S100B (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human melanoma. Anti-S100B (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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