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Pacific Blue Mouse IgG1, κ Isotype Control Antibody (S-844-79)

价格 1,790.00 供应商现货 : 3-5个工作日
货号 S0B1542
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产品规格
  • 宿主来源

    Mouse
  • 免疫原

    Recombinant Protein
  • 克隆号

    S-844-79
  • 抗体类型

    Mouse mAb
  • 纯化方式

    Protein G
  • 浓度

    0.2 mg/ml
  • 标记

    Pacific Blue
  • 性状

    Liquid
  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
  • 应用

    FCM
背景介绍
  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling SDT Pacific Blue Mouse IgG1, κ Isotype Control Antibody at 1 μg/test (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SDT Pacific Blue Mouse IgG1, κ Isotype Control Antibody at 1 μg/test (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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