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GSPT1 Recombinant Rabbit mAb (S-1914-49)

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A,Eukaryotic peptide chain release factor subunit 3a,eRF3a,G1 to S phase transition protein 1 homolog

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1483

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

GSPT1
分子别名

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P15170
克隆号

S-1914-49
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, HepG2, 293T, NIH/3T3, Neuro-2a, PC-12, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:3000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:3000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt

背景介绍

GSPT1, also known as eukaryotic release factor 3a (eRF3a), is a GTP-binding protein that plays a crucial role in both translation termination and cell cycle regulation. It functions by binding with eRF1 to dissociate proteins from the ribosome after translation, thus regulating cell cycle progression from the G1 to S phase. GSPT1 is overexpressed in various cancers, including gastric, colorectal, lung, and breast cancers, making it a promising target for cancer therapy. Additionally, GSPT1 is involved in cytoskeleton organization and apoptosis. Its degradation through molecular glue degraders, such as CC-885, has been shown to exert strong antitumor activity.

免疫印迹
- WB result of GSPT1 Recombinant Rabbit mAb
  Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 85 kDa
- WB result of GSPT1 Recombinant Rabbit mAb
  Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 85 kDa
- WB result of GSPT1 Recombinant Rabbit mAb
  Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 85 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of GSPT1 in tumor tissue.
- Expression of GSPT1 in human tissue.
- Expression of GSPT1 in mouse & rat tissue.