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Mouse IL-6 Recombinant mAb (S-1630-38) STAR

Interleukin-6,B-cell hybridoma growth factor,Interleukin HP-1,Il6

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1278
规格
数量
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产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Mouse IL-6

  • 分子别名

    Interleukin-6; B-cell hybridoma growth factor; Interleukin HP-1; Il6

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted

  • Accession

    P08505

  • 克隆号

    S-1630-38

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB

  • 反应种属 ?

    Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Ms
IHC-P 1:2000 Ms, Rt
ICC 1:500 Ms
背景介绍

  • Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in the coordination of both the innate and acquired immune responses, acting as a key mediator in inflammation and immune regulation. IL-6 is primarily produced at sites of inflammation and is secreted into the serum, where it triggers a transcriptional inflammatory response through the interleukin-6 receptor, alpha. This cytokine is involved in the maturation of B cells, the production of acute phase proteins, and the regulation of T cell responses, including the promotion of Th2 and Th17 cell differentiation. IL-6 also has effects on hepatocytes, inducing the production of C-reactive protein and other acute phase reactants, and it plays a role in the regulation of iron and zinc metabolism during inflammation. Additionally, IL-6 has been implicated in the pathogenesis of various diseases, including autoimmune disorders and cancer, where it can promote cell proliferation, differentiation, and apoptosis. The cytokine's signaling is mediated through the IL-6 receptor complex, which includes the IL-6R and the signaling subunit gp130, initiating intracellular signaling pathways such as JAK/STAT and ras/MAP kinase. IL-6's pleiotropic effects make it a significant target for therapeutic interventions in immunoinflammatory diseases.

  • 免疫印迹

    • WB result of mouse IL-6 Recombinant Rabbit mAb
      Primary antibody: mouse IL-6 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated RAW264.7 whole cell lysate 20 µg
      Lane 2: RAW264.7 treated with 100 ng/ml LPS for 4 hours and 1000 ng/ml Brefeldin A for 3 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 24 kDa
      Observed MW: 22 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Mouse IL-6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-Mouse IL-6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of Raw264.7 cells treated with LPS (100 ng/ml, 6h) add Brefeldin A (300 ng/ml, 3 h) (top panel) and untreated Raw264.7 cells (below panel). Anti- Mouse IL-6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Mouse IL-6 in mouse & rat tissue.

  • 斯达特公司的抗体，可以回收利用几次？

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