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宿主来源
Mouse抗原名称
CD133分子别名
Prominin-1; Antigen AC133; Prominin-like protein 1; PROM1; PROML1细胞定位
Apical cell membraneAccession
O43490克隆号
S-R586抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:200 | Hu |
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein that plays a role in cell signaling, cytoskeleton rearrangement, and protein trafficking, and is associated with stemness, cell proliferation, migration, and survival, particularly in the context of cancer stem cells and tumorigenesis.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Human CD133 antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45 - PE/Cy7 separately. Gated on total viable cells.
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