热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/12）
申请试用

Peroxiredoxin 1/PAG Recombinant Rabbit mAb (S-1673-22)

Natural killer cell-enhancing factor A (NKEF-A),Proliferation-associated gene protein (PAG),Thioredoxin peroxidase 2,Thioredoxin-dependent peroxide reductase 2,Thioredoxin-dependent peroxiredoxin 1,PRDX1,PAGA,PAGB,TDPX2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1184
规格
数量
收藏 分享

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Peroxiredoxin 1/PAG

  • 分子别名

    Natural killer cell-enhancing factor A (NKEF-A); Proliferation-associated gene protein (PAG); Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1; PRDX1; PAGA; PAGB; TDPX2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Melanosome

  • Accession

    Q06830

  • 克隆号

    S-1673-22

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    HeLa, HepG2, A431, A549, MCF7, K562, NIH/3T3, C2C12, mouse kidney

  • 预测反应种属
    (反应种属缩写表)

    Bv, Hm

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:10000 Hu, Ms
    IHC-P 1:1000 Hu, Ms
    ICC 1:500 Hu
背景介绍

  • Peroxiredoxin 1 (PRDX1) is a member of the peroxiredoxin family of antioxidant enzymes, which are ubiquitously distributed in all species and capable of reducing a broad range of peroxide substrates, including hydrogen peroxide (H2O2) and alkyl hydroperoxides. PRDX1 plays a crucial role in cellular redox homeostasis and is involved in the regulation of various cellular processes such as proliferation, differentiation, and apoptosis. PRDX1 has been implicated in the development and progression of various human cancers, including breast, esophageal, lung, and prostate cancers, where it regulates ROS-dependent signaling pathways and is thought to be a key intracellular intermediate balancing cell survival and apoptosis.

  • 免疫印迹

    • WB result of Peroxiredoxin 1/PAG Recombinant Rabbit mAb
      Primary antibody: Peroxiredoxin 1/PAG Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: A431 whole cell lysate 20 µg
      Lane 4: A549 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg
      Lane 6: K562 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
      Observed MW: 22 kDa

    • WB result of Peroxiredoxin 1/PAG Recombinant Rabbit mAb
      Primary antibody: Peroxiredoxin 1/PAG Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: C2C12 whole cell lysate 20 µg
      Lane 3: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
      Observed MW: 22 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse lung. Anti- Peroxiredoxin 1/PAG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Peroxiredoxin 1/PAG antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Peroxiredoxin 1/PAG in tumor tissue.

    • Expression of Peroxiredoxin 1/PAG in human tissue.

    • Expression of Peroxiredoxin 1/PAG in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)