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宿主来源
Rabbit抗原名称
Glutathione Peroxidase 1分子别名
GPx-1; GSHPx-1; Cellular glutathione peroxidase; Phospholipid-hydroperoxide glutathione peroxidase GPX1免疫原
Synthetic PeptideAccession
P07203克隆号
S-1714-17抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
THP-1, HL-60, mouse liver, mouse kidney, rat liver, rat kidney预测反应种属
(反应种属缩写表)Cz, Mq, Pg, Or, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Glutathione Peroxidase 1 (GPx1) is a crucial cellular antioxidant enzyme that plays a significant role in various biological processes. GPx1 is found in the cytoplasm and mitochondria of mammalian cells and is responsible for the enzymatic reduction of hydrogen peroxide and soluble lipid hydroperoxides using glutathione (GSH) as a source of reducing equivalents. Like most selenoenzymes, GPx1 contains a single redox-sensitive selenocysteine amino acid that is important for its function. Selenium availability and modifiers of selenocysteine incorporation can alter GPx1 expression, which in turn can promote disease states. As an antioxidant enzyme, GPx1 modulates the balance between necessary and harmful levels of ROS. It influences the thiol redox state and maintains the balance between necessary and harmful levels of cellular oxidants. GPx1 has been shown to have protective effects against various neurodegenerative diseases such as Parkinson's, Alzheimer's, brain ischemia, and seizure disorders. It may also play a role in neuropsychiatric conditions like stress, bipolar disorder, schizophrenia, and drug intoxication. GPx1 is involved in the innate immune response to lipopolysaccharide (LPS), protecting the lungs from oxidative damage. It is also implicated in the production of pro-inflammatory cytokines following an LPS attack. Furthermore, GPx1 plays a role in cardiovascular and metabolic health and is considered protective against the development and progression of many chronic diseases.
免疫印迹
WB result of Glutathione Peroxidase 1 Recombinant Rabbit mAb
Primary antibody: Glutathione Peroxidase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 20 kDaWB result of Glutathione Peroxidase 1 Recombinant Rabbit mAb
Primary antibody: Glutathione Peroxidase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 18 kDaWB result of Glutathione Peroxidase 1 Recombinant Rabbit mAb
Primary antibody: Glutathione Peroxidase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Glutathione Peroxidase 1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti- Glutathione Peroxidase 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Glutathione Peroxidase 1 in tumor tissue.
Expression of Glutathione Peroxidase 1 in human tissue.
Expression of Glutathione Peroxidase 1 in mouse & rat tissue.
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