CAD/BM1 Recombinant Rabbit mAb (S-1650-21)

Multifunctional protein CAD,Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1052

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CAD/BM1
分子别名

Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P27708
克隆号

S-1650-21
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, PC-3, HeLa, SW620, C2C12, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu, Ms, Rt
FCM 1:500

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Hu, Ms, Rt
FCM	1:500

背景介绍

The CAD protein, also known as carbamoyl phosphate synthetase II, is a multifunctional enzyme that plays a crucial role in the initiation of de novo pyrimidine biosynthesis in mammalian cells. It is a key regulatory enzyme involved in the synthesis of pyrimidines, which are essential components of nucleic acids. The CAD protein is composed of multiple domains that catalyze the first three steps of pyrimidine synthesis: carbamoyl phosphate synthesis, dihydroorotase, and aspartate transcarbamoylase activities. The allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle, and its activity is influenced by various effectors and subunit interactions. Moreover, the CAD protein has been implicated in coronary artery disease (CAD) through the integration of GWAS data and human plasma proteomes.

免疫印迹
- WB result of CAD/BM1 Recombinant Rabbit mAb
  Primary antibody: CAD/BM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: PC-3 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: SW620 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 243 kDa
  Observed MW: 260 kDa
- WB result of CAD/BM1 Recombinant Rabbit mAb
  Primary antibody: CAD/BM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 243 kDa
  Observed MW: 260 kDa
- WB result of CAD/BM1 Recombinant Rabbit mAb
  Primary antibody: CAD/BM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 243 kDa
  Observed MW: 260 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling CAD/BM1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CAD/BM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-CAD/BM1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-CAD/BM1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of CAD/BM1 in tumor tissue
- Expression of CAD/BM1 in human tissue
- Expression of CAD/BM1 in mouse & rat tissue