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CD48 Recombinant Rabbit mAb (S-1515-9)

CD48 antigen,B-lymphocyte activation marker BLAST-1,BCM1 surface antigen,Leukocyte antigen MEM-102,SLAM family member 2 (SLAMF2),Signaling lymphocytic activation molecule 2,TCT.1,BCM1,BLAST1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1051

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

CD48
分子别名

CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2 (SLAMF2); Signaling lymphocytic activation molecule 2; TCT.1; BCM1; BLAST1
免疫原

Recombinant Protein
细胞定位

Secreted, Cell membrane
Accession

P09326
克隆号

S-1515-9
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

Raji, Ramos
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:500	Hu

背景介绍

CD48 is a protein that belongs to the signal lymphocyte activation molecule (SLAMF) family. It is a cell-surface glycoprotein that plays a significant role in immune cell adhesion and activation. CD48 is expressed on various hematopoietic cells, including T cells, B cells, natural killer (NK) cells, monocytes, and eosinophils. CD48 does not have an intracellular domain; instead, it is anchored to the cell surface via a glycosyl-phosphatidyl-inositol (GPI) linkage. This protein interacts with several ligands, including CD2, CD244 (2B4), and bacterial FimH, to mediate various immune functions. The interaction of CD48 with CD2 on T cells can promote T cell activation by facilitating the recruitment of signaling components to the T cell receptor (TCR). CD48 also contributes to the organization of the immune synapse, adhesion, and costimulation through its interaction with CD2. The interaction between CD48 and CD244 can regulate the activation of NK cells and CD8+ T effector cells. CD48 also plays a role in the immune response against bacteria by binding to the bacterial component FimH through mannose residues in the GPI linkage. In terms of cancer treatment, CD48 has been identified as a potential target in immunotherapy. It is highly expressed in certain types of cancer, such as renal clear cell carcinoma (ccRCC), and may be associated with tumor immune evasion.

免疫印迹
- WB result of CD48 Recombinant Rabbit mAb
  Primary antibody: CD48 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: Ramos whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 28 kDa
  Observed MW: 38~45 kDa
免疫沉淀
- CD48 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD48 in 0.4 mg Ramos whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD48 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Ramos whole cell lysate 20 µg (Input)
  Lane 2: CD48 Rabbit mAb IP in Ramos whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Ramos whole cell lysate
  Predicted MW: 28 kDa
  Observed MW: 38~45 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of CD48 in tumor tissue
- Expression of CD48 in human tissue