RNA Polymerase II Recombinant Rabbit mAb (S-969-102)

DNA-directed RNA polymerase II subunit RPB1,RNA polymerase II subunit B1,3'-5' exoribonuclease,DNA-directed RNA polymerase II subunit A,DNA-directed RNA polymerase III largest subunit,RNA-directed RNA polymerase II subunit RPB1,POLR2A,POLR2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1039

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

RNA Polymerase II
分子别名

DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P24928
克隆号

S-969-102
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ChIP, ICC, WB, IP
反应种属 ?

Hu, Ms, Rt
阳性样本

293T, HeLa, MCF7, HCT 116, NIH/3T3, RAW264.7, C2C12, C6
预测反应种属
(反应种属缩写表)

Ba
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

RNA Polymerase II (RNAP II) is a crucial enzyme in eukaryotic cells responsible for transcribing protein-coding genes into mRNA and some non-coding RNAs. The function of RNAP II is to catalyze the transcription of DNA into RNA, which is a critical step in gene expression. The process of transcription can be divided into three stages: initiation, elongation, and termination. During initiation, RNAP II binds to the promoter region of a gene, assisted by various transcription factors, and begins to synthesize RNA. Elongation follows, where RNAP II moves along the DNA template, synthesizing the RNA chain. Termination occurs when RNAP II reaches a stop signal, releasing the newly synthesized RNA and disengaging from the DNA template. RNAP II plays a significant role in the regulation of transcription. It works in conjunction with a set of general transcription factors (GTFs), including TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH, to coordinate the transcription process. These factors dynamically associate with and dissociate from RNAP II to regulate the occurrence of transcription. RNAP II is also involved in the processing of mRNA, such as capping, splicing, and polyadenylation, through interactions with various factors during these modifications. Additionally, the CTD of RNAP II can undergo a series of modifications, such as phosphorylation, which provides a rapid pathway for plants to activate primary immune responses in the event of pathogen invasion.

免疫印迹
- WB result of RNA Polymerase II Recombinant Rabbit mAb
  Primary antibody: RNA Polymerase II Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: HCT 116 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 217 kDa
  Observed MW: 180~250 kDa
- WB result of RNA Polymerase II Recombinant Rabbit mAb
  Primary antibody: RNA Polymerase II Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Lane 3: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 217 kDa
  Observed MW: 180~250 kDa
- WB result of RNA Polymerase II Recombinant Rabbit mAb
  Primary antibody: RNA Polymerase II Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 217 kDa
  Observed MW: 180~250 kDa
免疫沉淀
- RNA Polymerase II Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating RNA Polymerase II in 0.4 mg 293T whole cell lysate.
  Western blot was performed on the immunoprecipitate using RNA Polymerase II Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: 293T whole cell lysate 40 µg (Input)
  Lane 2: RNA Polymerase II Recombinant Rabbit mAb IP in 293T whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
  Predicted MW: 217 kDa
  Observed MW: 180~250 kDa
  Exposure time: 180 s
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- RNA Polymerase II antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used RNA Polymerase II Recombinant Rabbit mAb (S-969-102) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of GAPDH, AFM and SAT-α in RNA Polymerase II Recombinant Rabbit mAb (S-969-102) - immunoprecipitated sample.