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RUVBL1 Recombinant Rabbit mAb (S-1600-38)

RuvB-like 1,49 kDa TATA box-binding protein-interacting protein (49 kDa TBP-interacting protein),54 kDa erythrocyte cytosolic protein (ECP-54),INO80 complex subunit H,Nuclear matrix protein 238 (NMP 238),Pontin 52,TIP49a,TIP60-associated protein 54-alpha (TAP54-alpha),INO80H,NMP238,TIP49

货号： S0B1030

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    RUVBL1

  • 分子别名

    RuvB-like 1; 49 kDa TATA box-binding protein-interacting protein (49 kDa TBP-interacting protein); 54 kDa erythrocyte cytosolic protein (ECP-54); INO80 complex subunit H; Nuclear matrix protein 238 (NMP 238); Pontin 52; TIP49a; TIP60-associated protein 54-alpha (TAP54-alpha); INO80H; NMP238; TIP49

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q9Y265

  • 克隆号

    S-1600-38

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    293T, A431, SK-MEL-28, PANC-1, HeLa, K562, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu
背景介绍

  • RUVBL1, also known as TIP49 or Pontin, is a protein that belongs to the AAA+ ATPase family. It forms a complex with RUVBL2, collectively known as RUVBL1/2, and functions as a co-chaperone in various cellular processes. These processes include chromatin remodeling, gene expression regulation, DNA replication, and the assembly and maturation of macromolecular complexes such as RNA polymerases and the mTOR complex. RUVBL1 has been implicated in cancer, with its overexpression associated with poor survival rates in various types of tumors. In non-small cell lung cancer (NSCLC), RUVBL1/2 ATPase activity is required for DNA replication and radioresistance, suggesting that inhibiting this activity could potentially increase the effectiveness of radiation therapy. Recent research has shown that RUVBL1 is involved in the regulation of the non-homologous end joining (NHEJ) pathway, which is a critical DNA repair mechanism. In head and neck squamous cell carcinoma (HNSCC), RUVBL1 is amplified and overexpressed, promoting proliferation and inhibiting the differentiation program. It plays a role in incorporating the histone variant H2AZ into chromatin, thereby regulating the transcription of key genes involved in differentiation, cancer cell proliferation, and invasion. Furthermore, RUVBL1 has been shown to correlate with the expression of molecular chaperones in hepatocellular carcinoma (HCC), and its activity is required for the stress response mediated by HSF1 (heat shock factor 1).

  • 免疫印迹

    • WB result of RUVBL1 Recombinant Rabbit mAb
      Primary antibody: RUVBL1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Lane 3: SK-MEL-28 whole cell lysate 20 µg
      Lane 4: PANC-1 whole cell lysate 20 µg
      Lane 5: HeLa whole cell lysate 20 µg
      Lane 6: K562 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 50 kDa

    • WB result of RUVBL1 Recombinant Rabbit mAb
      Primary antibody: RUVBL1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 50 kDa

    • WB result of RUVBL1 Recombinant Rabbit mAb
      Primary antibody: RUVBL1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 50 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) labelling RUVBL1 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-RUVBL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-RUVBL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-RUVBL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-RUVBL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-RUVBL1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in A431 cells. Anti- RUVBL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

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