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Rabbit抗原名称
Superoxide Dismutase 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P00441克隆号
S-1537-39抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, HEK-293, RAW264.7, rat liver预测反应种属
(反应种属缩写表)Or, CaMk, Pr, Hr, Cm, GP纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,ICC ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:10000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu, Ms
Superoxide Dismutase 1 (SOD1) is an essential enzyme found in the cells of nearly all living organisms. It plays a critical role in protecting cells against oxidative stress by catalyzing the dismutation of superoxide radicals into molecular oxygen and hydrogen peroxide. This process helps to prevent the damaging effects of reactive oxygen species (ROS), which can lead to cellular damage and are implicated in various diseases, including cancer, Alzheimer's, and amyotrophic lateral sclerosis (ALS). SOD1 is particularly important in the mitochondria, where it helps to maintain the balance between the production of energy and the generation of potentially harmful byproducts. The enzyme is encoded by the SOD1 gene and is a homodimeric metalloprotein, with each monomer containing a copper ion and a zinc ion at its active site. Mutations in the SOD1 gene have been linked to the development of familial ALS, highlighting the importance of this enzyme in maintaining cellular health.
免疫印迹
WB result of Superoxide Dismutase 1 Recombinant Rabbit mAb
Primary antibody: Superoxide Dismutase 1 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDaWB result of Superoxide Dismutase 1 Recombinant Rabbit mAb
Primary antibody: Superoxide Dismutase 1 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDaWB result of Superoxide Dismutase 1 Recombinant Rabbit mAb
Primary antibody: Superoxide Dismutase 1 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Superoxide Dismutase 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Superoxide Dismutase 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Raw 264.7 cells. Anti- Superoxide Dismutase 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Superoxide Dismutase 1 in tumor tissue.
Expression of Superoxide Dismutase 1 in human tissue.
Expression of Superoxide Dismutase 1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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