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Rat抗原名称
TER-119/Erythroid cells分子别名
Lymphocyte antigen 76; Ly76; Ly-76; Ter119细胞定位
Cell membrane克隆号
TER-119抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,FCM稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Ms FCM 1:2000 Ms
The TER-119 protein is one of the most specific and important cell surface markers for the murine erythroid lineage. It specifically recognizes an antigen associated with glycophorin A on the red blood cell membrane. Throughout erythroid development, from early basophilic erythroblasts to late-stage reticulocytes, it is consistently highly expressed, making it a critical molecular marker for identifying, sorting, and studying murine erythroid cells (such as erythrocytes, progenitor cells, and precursors). This protein belongs to the Ly-6 antigen superfamily, and its antibody (e.g., the TER-119 monoclonal antibody) is an indispensable tool in experimental hematology for the isolation and identification of red blood cells via flow cytometry or immunomagnetic beads, as well as for research into blood diseases like anemia and leukemia.
流式分析
Flow cytometric analysis of C57BL/6 mouse bone marrow labeling Mouse TER-119/Erythroid Cells at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45 - PE/Cy7 separately. Gated on total viable cells.
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Mouse TER-119/Erythroid Cells in tumor tissue.
Expression of Mouse TER-119/Erythroid Cells in human tissue.
Expression of Mouse TER-119/Erythroid Cells in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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