大包装询价 产品介绍 评论(0)
宿主来源
Rabbit抗原名称
UBASH3B/Sts1分子别名
Ubiquitin-associated and SH3 domain-containing protein B; Cbl-interacting protein p70; Suppressor of T-cell receptor signaling 1 (STS-1); T-cell ubiquitin ligand 2 (TULA-2); Tyrosine-protein phosphatase STS1/TULA2; KIAA1959免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q8TF42克隆号
S-1307-5抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:2000 |
| IP | 1:50 |
| IHC-P | 1:200 |
| ICC | 1:100 |
UBASH3B, also known as T-cell ubiquitin ligand 2 or suppressor of T-cell receptor signaling 1 (STS-1), is an atypical protein tyrosine phosphatase with a ubiquitin-binding domain. It plays a role in various cellular processes, including cell signaling, immune responses, and cellular development. In the context of cancer, UBASH3B has been implicated in tumorigenesis and cancer progression. Its abnormal expression is associated with the worsening of various cancers, and it may serve as a potential therapeutic target for inhibiting cancer growth. UBASH3B is also being investigated as a prognostic biomarker in prostate cancer, where its expression levels correlate with immune cell infiltration and overall prognosis. Furthermore, UBASH3B is involved in the regulation of immune responses, and its dysfunction has been linked to autoimmune diseases. It is expressed primarily in T-cells and has been associated with the regulation of T-cell receptor signaling.
免疫印迹
WB result of UBASH3B/Sts1 Recombinant Rabbit mAb
Primary antibody: UBASH3B/Sts1 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 68 kDa
免疫沉淀
UBASH3B/Sts1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating UBASH3B/Sts1 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using UBASH3B/Sts1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: UBASH3B/Sts1 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 73 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- UBASH3B/Sts1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti- UBASH3B/Sts1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- UBASH3B/Sts1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells (top panel) and negative staining in K562 cells (below panel). Anti-UBASH3B/Sts1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
评论(0)