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宿主来源
Rat抗原名称
CD20分子别名
B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1, Ms4a1, Ly-44, Ms4a2细胞定位
Cell membraneAccession
P19437克隆号
S-R497抗体类型
Rat mAb抗体同种型
Rat IgG2b,k应用
FCM反应种属 ?
Ms纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| FCM | 1:100 |
Mouse CD20, similar to its human counterpart, is a B cell-specific surface antigen that plays a role in B cell activation and is involved in the regulation of transmembrane calcium conductance. It is first expressed during the late pre-B cell stage of development and its expression increases as B cells mature in the bone marrow. CD20 expression is initiated at the transition from pre-B cells to immature B cells and continues to be expressed on mature B cells, with the exception of plasma cells. In mice, CD20 expression is B cell restricted and can be used as a marker for certain B cell subsets, including transitional T1 B cells in the spleen, which also express high levels of CD20. This makes CD20 a useful marker for studying these cells. The frequency and density of CD20 increase during B cell maturation, with a subpopulation of transitional IgMhi B cells showing higher CD20 levels than most mature recirculating B cells.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Mouse CD20 at 1/100 (2 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Alexa Fluor® 647 separately. Gated on total viable cells.
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