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UGGT1 Recombinant Rabbit mAb (S-1079-19)

UDP-glucose:glycoprotein glucosyltransferase 1,UGT1,hUGT1,UDP--Glc:glycoprotein glucosyltransferase,UDP-glucose ceramide glucosyltransferase-like 1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0774

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

UGGT1
分子别名

UDP-glucose:glycoprotein glucosyltransferase 1; UGT1; hUGT1; UDP--Glc:glycoprotein glucosyltransferase; UDP-glucose ceramide glucosyltransferase-like 1
免疫原

Synthetic Peptide
细胞定位

Endoplasmic reticulum
Accession

Q9NYU2
克隆号

S-1079-19
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:500 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:500	Hu, Ms, Rt

背景介绍

UGGT1, known as UDP-Glucose Glycoprotein Glucosyltransferase 1, is an enzyme involved in the process of protein glycosylation. It is a soluble protein in the endoplasmic reticulum (ER) that selectively reglycosylates unfolded glycoproteins, providing quality control for protein trafficking out of the ER. In the process of hepatitis C virus (HCV) entry into cells, UGGT1 has been identified as a host factor that affects HCV entry by influencing SR-BI. As a biomarker, UGGT1 has potential applications in the diagnosis and treatment of cervical diseases such as cervical intraepithelial neoplasia and cervical squamous cell carcinoma.

免疫印迹
- WB result of UGGT1 Recombinant Rabbit mAb
  Primary antibody: UGGT1 Recombinant mAb at 1/1000 dilution
  Lane 1: SH-SY5Y whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: MCF-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 177 kDa
  Observed MW: 170 kDa
免疫沉淀
- UGGT1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating UGGT1 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using UGGT1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat whole cell lysate 5 µg (Input)
  Lane 2: UGGT1 Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 177 kDa
  Observed MW: 170 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学