Human IgG4 Recombinant Rabbit mAb (S-1140-53)

Immunoglobulin heavy constant gamma 4,Ig gamma-4 chain C region,IGHG4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0767

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Human IgG4
分子别名

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
免疫原

Synthetic Peptide
细胞定位

Secreted
Accession

P01861
克隆号

S-1140-53
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ? ,
ELISA ,
WB ,
IF ?
稀释度

应用稀释度

WB 1:1000
IHC-P 1:2000
IF 1:2000

应用	稀释度
WB	1:1000
IHC-P	1:2000
IF	1:2000

背景介绍

IgG4 is a subclass of immunoglobulin G (IgG), which comprises four main subclasses: IgG1, IgG2, IgG3, and IgG4. IgG4 primarily participates in immune regulation within the immune system and plays a significant role in certain inflammatory responses. For instance, it can form cross-links with other antibodies, preventing them from binding to antigens, thereby reducing the intensity of inflammatory reactions. Elevated IgG4 levels are often observed in autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and Sjögren's syndrome.

免疫印迹
- WB result of Human IgG4 Recombinant Rabbit mAb
  Primary antibody: Human IgG4 Recombinant mAb at 1/1000 dilution
  Lane 1: human serum lysate 5 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 50 kDa
- WB result of Human IgG4 Recombinant Rabbit mAb
  Primary antibody: Human IgG4 Recombinant mAb at 1/1000 dilution
  Lane 1: IgG1 FC recombinant protein lysate 10 ng
  Lane 2: IgG2 FC recombinant protein lysate 10 ng
  Lane 3: IgG3 FC recombinant protein lysate 10 ng
  Lane 4: IgG4 FC recombinant protein lysate 10 ng
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 35 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.