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Mouse免疫原
Recombinant Protein克隆号
S-844-28抗体类型
Mouse mAb应用
IHC-P, FCM, ICC, WB浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
免疫印迹
WB result of Mouse mAb IgM, κ Isotype Control
Primary antibody: Mouse mAb IgM, κ Isotype Control at 1/100 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
流式分析
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Mouse mAb IgM, κ Isotype Control at 1/20 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with Mouse mAb IgM, κ Isotype Control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows negative staining in paraffin-embedded human tonsil. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded rat spleen. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows negative staining in C6 cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows negative staining in HeLa cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
ICC shows negative staining in C2C12 cells. Mouse mAb IgM, κ Isotype Control was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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