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Runx3 Recombinant Rabbit mAb (S-R356)

Runt-related transcription factor 3,Acute myeloid leukemia 2 protein,Core-binding factor subunit alpha-3 (CBF-alpha-3),Oncogene AML-2,Polyomavirus enhancer-binding protein 2 alpha C subunit (PEA2-alpha C,PEBP2-alpha C),SL3-3 enhancer factor 1 alpha C subunit,SL3/AKV core-binding factor alpha C subunit,AML2,CBFA3,PEBP2A3

货号： S0B0487

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3 (CBF-alpha-3), Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit (PEA2-alpha C; PEBP2-alpha C), SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, AML2, CBFA3, PEBP2A3

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q13761

  • 克隆号

    S-R356

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500
IHC-P 1:500
IP 1:50
背景介绍

  • Runx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters,[6] and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.

  • 免疫印迹

    • WB result of Runx3 Rabbit mAb
      Primary antibody: Runx3 Rabbit mAb at 1/500 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: SW620 whole cell lysate 20 µg
      Negative control: MCF7 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 48 kDa
      (This blot was developed with high sensitivity substrate)

  • 免疫沉淀

    • Runx3 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Runx3 in 0.4 mg SW620 whole cell lysate.
      Western blot was performed on the immunoprecipitate using Runx3 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: SW620 whole cell lysate 10 µg (Input)
      Lane 2: Runx3 Rabbit mAb IP in SW620 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in SW620 whole cell lysate
      Predicted MW: 44 kDa
      Observed MW: 48 kDa
      （This blot was developed with high sensitivity substrate）

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human thymus. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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