α-Synuclein Recombinant Rabbit mAb (S-441-86)

Non-A beta component of AD amyloid,Non-A4 component of amyloid precursor (NACP),SNCA,NACP,PARK1,alpha-Synuclein,α-Syn

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0344

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

α-Synuclein
分子别名

Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP), SNCA, NACP, PARK1, alpha-Synuclein; α-Syn
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus, Membrane
Accession

P37840
克隆号

S-441-86
抗体类型

Recombinant mAb
应用

IHC-P ? ,
WB ,
IP ,
IF ?
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50
IHC	1:2000
IF	1:1000

背景介绍

Alpha-synuclein is a neuronal protein that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. It is abundant in the brain, while smaller amounts are found in the heart, muscle and other tissues. In the brain, alpha-synuclein is found mainly in the axon terminals of presynaptic neurons. Within these terminals, alpha-synuclein interacts with phospholipids and proteins. Presynaptic terminals release chemical messengers, called neurotransmitters, from compartments known as synaptic vesicles. The release of neurotransmitters relays signals between neurons and is critical for normal brain function.

免疫印迹
- WB result of α-Synuclein Rabbit mAb
  Primary antibody: α-Synuclein Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 18 kDa
- WB result of α-Synuclein Rabbit mAb
  Primary antibody: α-Synuclein Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 18 kDa
免疫沉淀
- α-Synuclein Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating α-Synuclein in 0.4 mg mouse brain lysate.
  Western blot was performed on the immunoprecipitate using α-Synuclein Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: mouse brain lysate 20 µg (Input)
  Lane 2: α-Synuclein Rabbit mAb IP in mouse brain lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse brain lysate
  Predicted MW: 14 kDa
  Observed MW: 18 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse testis. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse brain. Anti-α-Synuclein antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.