Rabbit mAb IgG Isotype Control (S-240-1)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0276

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
免疫原

Recombinant Protein
克隆号

S-240-1
抗体类型

Rabbit mAb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
FCM ,
WB ,
IP

背景介绍

Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

免疫印迹
- WB result of Rabbit mAb IgG Isotype Control Primary antibody: Rabbit mAb IgG Isotype Control at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
- WB result of Rabbit mAb IgG Isotype Control Primary antibody: Rabbit mAb IgG Isotype Control at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Lane 2: mouse testis lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
- WB result of Rabbit mAb IgG Isotype Control Primary antibody: Rabbit mAb IgG Isotype Control at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Lane 2: rat testis lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
流式分析
- Flow cytometric analysis of mouse primary splenocytes labeling Rabbit IgG isotype control at 1/50 dilution (1 μg) / (left panel) compared with CD8α antibody at 1/50 (1 μg) dilution (S0B0034) / (right panel). Goat Anti-Rabbit IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 - Alexa Fluor 647 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling Rabbit IgG isotype control at 1/50 dilution (1 μg) / (left panel) compared with Stat6 antibody at 1/50 (1 μg) dilution (S0B0085) / (right panel). Goat Anti-Rabbit IgG Alexa Fluor 488 was used as the secondary antibody.
免疫沉淀
- SPARC Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SPARC in 0.4 mg A375 whole cell lysate.
  Western blot was performed on the immunoprecipitate using SPARC Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: A375 whole cell lysate 20 µg (Input)
  Lane 2: SPARC Rabbit mAb IP in A375 whole cell lysate
  Lane 3: Rabbit mAb IgG Isotype Control IP in A375 whole cell lysate
  Predicted MW: 35 kDa
  Observed MW: 37 kDa
- Ku80 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Ku80 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Ku80 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: Ku80 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit mAb IgG Isotype Control IP in HeLa whole cell lysate
  Predicted MW: 83 kDa
  Observed MW: 86 kDa
免疫组化
- IHC shows negative staining in paraffin-embedded human cerebral cortex. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human kidney. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human tonsil. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human breast cancer. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human colon cancer. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded rat spleen. Rabbit mAb IgG Isotype Control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.