F4/80 Recombinant Rabbit mAb (SDT-313-49)

浏览次数：94

货号： S0B0227

Datasheet COA

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

F4/80
分子别名

Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EMR1 hormone receptor, Gpf480, Adgre1
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

Q61549
克隆号

SDT-313-49
抗体类型

Recombinant mAb
应用

IHC-P, ICC, WB
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500
ICC	1:100

背景介绍

The F4/80 monoclonal antibody has been used widely as a marker for mouse macrophages [PMID: 16087400]. EMR1-F4/80 is mainly present in the macrophage-containing stroma-vascular fraction. Furthermore, fibroblasts-like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3-F442A and BFC-1, express CD14 and CD68 mRNA and protein as determined by fluorescence-activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7 [PMID: 16213494].

免疫印迹
- WB result of F4/80 Rabbit mAb
  Primary antibody: F4/80 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Negative control: NIH/3T3 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 97kDa
  Observed MW: 300kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Raw264.7 cells. Anti-F4/80 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in NIH/3T3 cells. Anti-F4/80 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

引用文献(1)

Inhibition of Drp1- Fis1 interaction alleviates aberrant mitochondrial fragmentation and acute kidney injury
Z Song, Y Xia, L Shi, H Zha, J Huang, X Xiang

Cellular & Molecular Biology Letters. 2024 Mar 04 .

影响因子： 8.3

货号：S0B0227产品名称：F4/80 Recombinant Rabbit mAb (SDT-313-49)