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宿主来源
Rabbit抗原名称
β-Catenin分子别名
Catenin beta-1, Beta-catenin，CTNNB1免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
P35222克隆号
S-336-23抗体类型
Recombinant mAb抗体同种型
IgG应用
IF反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:200 |
β-catenin is a multifunction protein with a central role in physiological homeostasis. Its abnormal expression leads to various diseases including cancer. In normal physiology, β-catenin either maintains integrity of epithelial tissues or controls transcription of various genes on extracellular instigations. In epithelial tissues, β-catenin functions as a component of the cadherin protein complex and regulates epithelial cell growth and intracellular adhesion [PMID: 23490077].
免疫荧光
IF shows positive staining in paraffin-embedded human prostate cancer. Anti-β-Catenin (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human thyroid cancer. Anti-β-Catenin (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti-β-Catenin (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti-β-Catenin (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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