Vimentin Recombinant Rabbit mAb (Alexa Fluor^® 488 Conjugate) (S-029-20)

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货号： S0B0141

大包装询价

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产品规格

稀释度

应用	稀释度
ICC	1:500
IF	1:500

背景介绍

Vimentin is a structural protein that in humans is encoded by the VIM gene. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Vimentin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in NIH/3T3 cells. Anti-Vimentin(Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C.The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in C6 cells. Anti-Vimentin(Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C.The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
- IF shows positive staining in paraffin-embedded human colon cancer. Anti- Vimentin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.