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Rabbit抗原名称
GLUT-1分子别名
SLC2A1, GLUT1免疫原
Synthetic Peptide细胞定位
Cell membrane, MelanosomeAccession
P11166克隆号
SDT-047-53抗体类型
Rabbit mAb应用
FCM ,ICC ?反应种属 ?
Hu预测反应种属
(反应种属缩写表)Ms, Rb, Bv, Rt, Pg, Sh纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
Glucose enters cells through various transporters including the GLUT family of facilitative transporters, the sodium/glucose co-transporters, and the recently discovered SWEET family. however, only the role of the GLUT family has been extensively studied in cancer cells. There are 14 GLUT proteins in the human and 12 in the mouse. GLUT1, encoded by SLC2A1, is the predominant transporter overexpressed in tumors, including hepatic, pancreatic, esophageal, brain, renal, lung, cutaneous, colorectal, endometrial, ovarian, cervical, and breast, as well as head and neck tumors.
流式分析
Flow cytometric analysis of 4% FPA fixed 90% methanol permeabilized HepG2 cells labelling GLUT-1 (Alexa Fluor 594 Conjugate) antibody at 1/200 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-GLUT1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
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