alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor^® 647 Conjugate) (SDT-132-63)

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货号： S0B0119

Datasheet COA

价格： ￥1,056
规格：
25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

alpha smooth muscle Actin (SMA)
分子别名

Alpha-actin-2,ACTA2
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

P62736
克隆号

SDT-132-63
抗体类型

Recombinant mAb
应用

ICFCM, ICC, IF
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

2 mg/ml
标记

Alexa Fluor® 647
性状

Liquid
缓冲体系

PBS, 0.1% BSA, 0.01% Proclin 300
储存条件

12 months from date of receipt / reconstitution, 4 °C as supplied.

稀释度

应用	稀释度
IF	1:1000
ICFCM	1:200
ICC	1:1000

背景介绍

α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

流式分析
- SDT-132_alpha smooth muscle Actin (Alexa Fluor® 647)_FC_1
  Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫荧光
- IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.