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Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)
规格：25μl
价格：￥120.00
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宿主来源
Rabbit抗原名称
β-actin分子别名
ACTB免疫原
N/A细胞定位
Cytoplasm, CytoskeletonAccession
P60709克隆号
SDT-R015抗体类型
Recombinant mAb应用
Functional Assay ,WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
4 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ICC | 1:100 |
| ICFCM | 1:400-1:4000 |
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
流式分析
Flow cytometric analysis of HeLa cells labelling β-actin (Alexa Fluor® 647 Conjugate) antibody at 1/4000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of NIH/3T3 cells labelling β-actin (Alexa Fluor® 647 Conjugate) antibody at 1/400 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of C6 cells labelling β-actin (Alexa Fluor® 647 Conjugate) antibody at 1/4000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-β-actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-β-actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti-β-actin (Alexa Fluor® 647 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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