GLUT2 Recombinant Rabbit mAb (SDT-R036)

SLC2A2

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0030

规格

25μl100μl1ml

数量

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产品规格

稀释度

应用	稀释度
WB	1:1000
ICC	1:100

背景介绍

GLUT2 transports both glucose and fructose with low affinity and plays a critical role in glucose sensing mechanisms. Alterations in the function or expression of GLUT2 are involved in the Fanconi–Bickel syndrome, diabetes, and cancer.

免疫印迹
- WB result of GLUT2 Rabbit mAb
  Primary antibody: GLUT2 Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 45~60 kDa
  Observed MW: 54 kDa
  Exposure time: 35s
- WB result of GLUT2 Rabbit mAb
  Primary antibody: GLUT2 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 45~60 kDa
  Observed MW: 54 kDa
  Exposure time: 35s
- WB result of GLUT2 Rabbit mAb
  Primary antibody: GLUT2 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 45~60 kDa
  Observed MW: 54 kDa
  Exposure time: 35s
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-GLUT2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).