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His tag Recombinant Rabbit mAb (SDT-R007)

价格 400.00 供应商现货 : 3-5个工作日
货号 S0B0006
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产品规格
  • 宿主来源

    Rabbit
  • 免疫原

    Synthetic Peptide
  • 克隆号

    SDT-R007
  • 抗体类型

    Rabbit mAb
  • 反应种属 ?

    Species Independent
  • 纯化方式

    Protein A
  • 浓度

    1mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

  • 应用

    WB
  • 稀释度

    应用 稀释度
    WB 1:1000-1:10000
背景介绍
  • A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.

  • 免疫印迹

    • WB result of His-tag Rabbit mAb
      Primary antibody : His-tag Rabbit mAb at 1/1000 dilution
      Lane 1: supernantant of Expi293F transfected with His tagged CGB3 10 μg
      Lane 2: purified recombinant His tagged IFN-α1 10 ng
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: His tagged CGB3: 17 kDa;
      His tagged IFN-α1: 21kDa
      Observed MW: His tagged CGB3: 34 kDa;
      His tagged IFN-α1: 21kDa
      Exposure time: 60s

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