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物种
HumanAccession
P05113表达序列
Ile20-Ser134, with N-His Tag
表达宿主
HEK293分子量
Predicted MW: 14.8 kDa Observed MW: 17-23 kDa
纯度
＞95% by SDS-PAGE标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
0.2M PBS, pH7.4 with 3% trehalose.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
IL-5 is the predominant cytokine associated with antigen-induced eosinophilic inflammation in the lung. The activation of Th-2 cells leads to the production of IL-5. The pro-eosinophilic effects of IL-5 include: (1) enhanced replication and differentiation of eosinophilic myelocytes; (2) enhanced degranulation of eosinophils; (3) prolonged survival time of eosinophils: and (4) enhanced adhesion of eosinophils. The effects of IL-5 are mediated via the interaction of IL-5 with receptors (IL-5R) that are expressed on the eosinophil cell membrane. Intracellular signalling produced by occupation of the IL-5R by IL-5 occurs via the JAK-STAT system.
电泳
2μg (R: reducing condition).
ELISA
Immobilized Human IL-5 Protein, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha / CD125 Protein, Fc Tag with EC50 of 13.4-16.6 ng/ml.
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