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物种
Human分子别名
IL21,Za11,Interleukin-21Accession
Q9HBE4-1表达序列
Gln 30 - Ser 162,with human IgG1 Fc tag at the C-terminus.
表达宿主
HEK293分子量
MW of 41.9 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.纯度
> 95% by SDS-PAGE内毒素含量
<1EU/μg活性
Immobilized Human IL-21 R, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-21, Fc Tag with a linear range of 0.2-8 ng/mL标记
Unconjugated性状
Lyophilized Powder缓冲体系
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
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