IGF-I Protein, Human

Insulin-Like Growth Factor I,IGF-I,Mechano Growth Factor,MGF,Somatomedin-C,IGF1,IBP1

价格￥200.00 （供应商现货： 3-5个工作日）

货号 UA100025

数量

产品介绍

产品介绍评论(0)

产品规格

物种

Human
分子别名

Insulin-Like Growth Factor I; IGF-I; Mechano Growth Factor; MGF; Somatomedin-C; IGF1; IBP1
Accession

QP05019
表达序列

Gly49-Ala118
表达宿主

E.coli
分子量

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
纯度

＞98% by SDS-PAGE or HPLC.
活性

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.
标记

Unconjugated
标签

No Tag
性状

Lyophilized Powder
缓冲体系

20 mM PBS, pH 7.0.
溶解方法

Before use this product, please read the direction below carefully.
This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in a sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
储存条件

For long term storage, the product should be stored ≤ -20℃.
Please avoid repeated freeze-thaw cycles after reconstitution.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
3 months, -20 to -70℃ under sterile conditions after reconstitution.

背景介绍

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively , and exhibits cross-species activity. Recombinant human IGF-1 is a non-glycosylated polypeptide chain containing 70 amino acids..